Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 160285..160875 Replicon chromosome
Accession NZ_CP063926
Organism Klebsiella variicola strain S187-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA69_RS00695 Protein ID WP_008804165.1
Coordinates 160543..160875 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA69_RS00690 Protein ID WP_012541132.1
Coordinates 160285..160542 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA69_RS00670 155894..156469 + 576 WP_077139353.1 hypothetical protein -
ISA69_RS00675 156646..157482 + 837 WP_008804155.1 alpha/beta hydrolase -
ISA69_RS00680 157688..158659 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA69_RS00685 158656..159756 - 1101 WP_048268588.1 phosphotransferase -
ISA69_RS00690 160285..160542 + 258 WP_012541132.1 antitoxin Antitoxin
ISA69_RS00695 160543..160875 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA69_RS00705 161198..162634 + 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA69_RS00715 163007..164461 - 1455 WP_008804170.1 AMP nucleosidase -
ISA69_RS00720 164592..164837 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179581 WP_008804165.1 NZ_CP063926:160543-160875 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179581 NZ_CP084843:c2744648-2743326 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCCTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCAGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179581 WP_012541132.1 NZ_CP063926:160285-160542 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179581 NZ_CP084843:c2744914-2744648 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References