Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 94059..94702 | Replicon | plasmid pBM338-1.2 |
| Accession | NZ_CP063924 | ||
| Organism | Klebsiella pneumoniae strain BM338-1 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | - |
| Locus tag | ISA71_RS27140 | Protein ID | WP_069985717.1 |
| Coordinates | 94286..94702 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A6B7Q5S4 |
| Locus tag | ISA71_RS27135 | Protein ID | WP_021312476.1 |
| Coordinates | 94059..94289 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ISA71_RS27095 | 89196..90212 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| ISA71_RS27100 | 90223..90537 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| ISA71_RS27105 | 90564..90959 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| ISA71_RS27110 | 91128..91433 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | - |
| ISA71_RS27115 | 91435..91653 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| ISA71_RS27120 | 91823..92089 | - | 267 | Protein_112 | hypothetical protein | - |
| ISA71_RS27125 | 92208..92897 | + | 690 | WP_157263246.1 | hypothetical protein | - |
| ISA71_RS27130 | 92929..93618 | - | 690 | WP_069985716.1 | RES family NAD+ phosphorylase | - |
| ISA71_RS27135 | 94059..94289 | + | 231 | WP_021312476.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| ISA71_RS27140 | 94286..94702 | + | 417 | WP_069985717.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| ISA71_RS27145 | 94748..98551 | - | 3804 | WP_181590013.1 | pcar | - |
| ISA71_RS27150 | 98899..99162 | + | 264 | WP_009310051.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1B / blaCTX-M-3 / qnrS1 | - | 1..116734 | 116734 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 14989.47 Da Isoelectric Point: 9.2738
>T179563 WP_069985717.1 NZ_CP063924:94286-94702 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK
Download Length: 417 bp
>T179563 NZ_CP084830:1934184-1934286 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 77 a.a. Molecular weight: 8723.76 Da Isoelectric Point: 4.3410
>AT179563 WP_021312476.1 NZ_CP063924:94059-94289 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL
Download Length: 231 bp
>AT179563 NZ_CP084830:c1934293-1934148 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|