Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 91128..91653 Replicon plasmid pBM338-1.2
Accession NZ_CP063924
Organism Klebsiella pneumoniae strain BM338-1

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag ISA71_RS27110 Protein ID WP_013023785.1
Coordinates 91128..91433 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag ISA71_RS27115 Protein ID WP_001568025.1
Coordinates 91435..91653 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA71_RS27080 86823..87449 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
ISA71_RS27085 87569..87748 + 180 WP_006788217.1 hypothetical protein -
ISA71_RS27090 88204..88998 - 795 WP_004197635.1 site-specific integrase -
ISA71_RS27095 89196..90212 - 1017 WP_017899884.1 hypothetical protein -
ISA71_RS27100 90223..90537 - 315 WP_053389906.1 hypothetical protein -
ISA71_RS27105 90564..90959 - 396 WP_017899885.1 hypothetical protein -
ISA71_RS27110 91128..91433 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
ISA71_RS27115 91435..91653 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISA71_RS27120 91823..92089 - 267 Protein_112 hypothetical protein -
ISA71_RS27125 92208..92897 + 690 WP_157263246.1 hypothetical protein -
ISA71_RS27130 92929..93618 - 690 WP_069985716.1 RES family NAD+ phosphorylase -
ISA71_RS27135 94059..94289 + 231 WP_021312476.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISA71_RS27140 94286..94702 + 417 WP_069985717.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..116734 116734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T179562 WP_013023785.1 NZ_CP063924:c91433-91128 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179562 NZ_CP084830:801347-801757 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT179562 WP_001568025.1 NZ_CP063924:c91653-91435 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179562 NZ_CP084830:801100-801366 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References