Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4308316..4308935 Replicon chromosome
Accession NZ_CP063922
Organism Klebsiella pneumoniae strain BM338-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA71_RS20990 Protein ID WP_002892050.1
Coordinates 4308316..4308534 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA71_RS20995 Protein ID WP_002892066.1
Coordinates 4308561..4308935 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA71_RS20955 4304363..4304626 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
ISA71_RS20960 4304626..4304766 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA71_RS20965 4304763..4305461 - 699 WP_004893822.1 GNAT family N-acetyltransferase -
ISA71_RS20970 4305562..4307013 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
ISA71_RS20975 4306988..4307458 - 471 WP_002892026.1 YlaC family protein -
ISA71_RS20980 4307479..4307619 + 141 WP_032409038.1 hypothetical protein -
ISA71_RS20985 4307591..4308157 - 567 WP_002892030.1 maltose O-acetyltransferase -
ISA71_RS20990 4308316..4308534 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA71_RS20995 4308561..4308935 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA71_RS21000 4309421..4312567 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA71_RS21005 4312590..4313783 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179556 WP_002892050.1 NZ_CP063922:c4308534-4308316 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179556 NZ_CP084829:c4529064-4528531 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179556 WP_002892066.1 NZ_CP063922:c4308935-4308561 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179556 NZ_CP084829:c4529341-4529075 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References