Detailed information of TA system TA179527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2514121..2514707
• Replicon: chromosome
• Accession: NZ_CP063921
• Organism: Klebsiella variicola strain BM366-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ISA73_RS11805
• Protein ID: WP_002920800.1
• Coordinates: 2514339..2514707 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1F2M0C2
• Locus tag: ISA73_RS11800
• Protein ID: WP_032731361.1
• Coordinates: 2514121..2514342 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA73_RS11780	2510278..2511204	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ISA73_RS11785	2511201..2512478	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ISA73_RS11790	2512475..2513242	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ISA73_RS11795	2513244..2513957	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ISA73_RS11800	2514121..2514342	+	222	WP_032731361.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ISA73_RS11805	2514339..2514707	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ISA73_RS11810	2514999..2516315	+	1317	WP_194386930.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ISA73_RS11815	2516422..2517309	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ISA73_RS11820	2517306..2518151	+	846	WP_032740290.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ISA73_RS11825	2518153..2519223	+	1071	WP_023323353.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2509107..2519960	10853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T179527 WP_002920800.1 NZ_CP063921:2514339-2514707 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T179527 NZ_CP084823:c568874-568389 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8165.18 Da
• Isoelectric Point: 4.7769

>AT179527 WP_032731361.1 NZ_CP063921:2514121-2514342 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT179527 NZ_CP084823:c569128-568862 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A1F2M0C2