Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 920341..920960 Replicon chromosome
Accession NZ_CP063921
Organism Klebsiella variicola strain BM366-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA73_RS04385 Protein ID WP_002892050.1
Coordinates 920742..920960 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA73_RS04380 Protein ID WP_008805436.1
Coordinates 920341..920715 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA73_RS04370 915496..916689 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISA73_RS04375 916712..919858 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA73_RS04380 920341..920715 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA73_RS04385 920742..920960 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA73_RS04390 921119..921685 + 567 WP_008805435.1 maltose O-acetyltransferase -
ISA73_RS04395 921657..921785 - 129 Protein_859 hypothetical protein -
ISA73_RS04400 921822..922292 + 471 WP_008805434.1 YlaC family protein -
ISA73_RS04405 922261..923718 - 1458 WP_194387274.1 PLP-dependent aminotransferase family protein -
ISA73_RS04410 923819..924517 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA73_RS04415 924514..924654 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA73_RS04420 924654..924917 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179524 WP_002892050.1 NZ_CP063921:920742-920960 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179524 NZ_CP084820:c53233-52817 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179524 WP_008805436.1 NZ_CP063921:920341-920715 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179524 NZ_CP084820:c53460-53230 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References