Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2589189..2589808 Replicon chromosome
Accession NZ_CP063919
Organism Klebsiella pneumoniae strain BM337-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA74_RS12465 Protein ID WP_002892050.1
Coordinates 2589189..2589407 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA74_RS12470 Protein ID WP_002892066.1
Coordinates 2589434..2589808 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA74_RS12430 2585236..2585499 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
ISA74_RS12435 2585499..2585639 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA74_RS12440 2585636..2586334 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
ISA74_RS12445 2586435..2587886 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
ISA74_RS12450 2587861..2588331 - 471 WP_002892026.1 YlaC family protein -
ISA74_RS12455 2588352..2588492 + 141 WP_004147370.1 hypothetical protein -
ISA74_RS12460 2588464..2589030 - 567 WP_002892030.1 maltose O-acetyltransferase -
ISA74_RS12465 2589189..2589407 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA74_RS12470 2589434..2589808 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA74_RS12475 2590294..2593440 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA74_RS12480 2593463..2594656 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179510 WP_002892050.1 NZ_CP063919:c2589407-2589189 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179510 NZ_CP084807:c10590-10285 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179510 WP_002892066.1 NZ_CP063919:c2589808-2589434 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179510 NZ_CP084807:c10810-10592 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References