Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1991179..1991989 Replicon chromosome
Accession NZ_CP063919
Organism Klebsiella pneumoniae strain BM337-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISA74_RS09590 Protein ID WP_004178461.1
Coordinates 1991456..1991989 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA74_RS09585 Protein ID WP_002887278.1
Coordinates 1991179..1991445 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA74_RS09565 1987050..1987394 - 345 WP_002887266.1 cation efflux system protein CusF -
ISA74_RS09570 1987412..1988797 - 1386 WP_032413315.1 efflux transporter outer membrane subunit -
ISA74_RS09575 1988970..1989653 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
ISA74_RS09580 1989643..1991076 + 1434 WP_032413318.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA74_RS09585 1991179..1991445 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA74_RS09590 1991456..1991989 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
ISA74_RS09595 1992037..1993158 - 1122 WP_016946712.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179509 WP_004178461.1 NZ_CP063919:1991456-1991989 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179509 NZ_CP084806:c4586128-4585889 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179509 WP_002887278.1 NZ_CP063919:1991179-1991445 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179509 NZ_CP084806:c4586360-4586118 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References