Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1402745..1403370 Replicon chromosome
Accession NZ_CP063919
Organism Klebsiella pneumoniae strain BM337-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISA74_RS06805 Protein ID WP_002882817.1
Coordinates 1402987..1403370 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA74_RS06800 Protein ID WP_004150355.1
Coordinates 1402745..1402987 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA74_RS06775 1398735..1399334 + 600 WP_004151865.1 glucose-1-phosphatase -
ISA74_RS06780 1399328..1400188 + 861 WP_004146232.1 virulence factor BrkB family protein -
ISA74_RS06785 1400185..1400622 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA74_RS06790 1400667..1401608 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISA74_RS06795 1401623..1402540 - 918 WP_012737090.1 alpha/beta hydrolase -
ISA74_RS06800 1402745..1402987 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA74_RS06805 1402987..1403370 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA74_RS06810 1403544..1404473 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA74_RS06815 1404470..1405105 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA74_RS06820 1405102..1406004 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179505 WP_002882817.1 NZ_CP063919:1402987-1403370 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179505 NZ_CP084806:775176-775586 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTACTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCATGGGTCATCAACAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATCCCGGAGAGTGGCGGGATCTGCGCCGGTTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179505 WP_004150355.1 NZ_CP063919:1402745-1402987 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179505 NZ_CP084806:774929-775195 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References