Detailed information of TA system TA179500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5008..5651
• Replicon: plasmid pBM374-1
• Accession: NZ_CP063918
• Organism: Klebsiella variicola strain BM374-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ISA75_RS26830
• Protein ID: WP_016946796.1
• Coordinates: 5008..5424 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: ISA75_RS26835
• Protein ID: WP_001261276.1
• Coordinates: 5421..5651 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA75_RS26805	884..2104	+	1221	WP_100632062.1	MFS transporter	-
ISA75_RS26810	2101..3342	+	1242	WP_100632063.1	ATP-grasp domain-containing protein	-
ISA75_RS26815	3339..4226	+	888	WP_148382199.1	ABC transporter substrate-binding protein	-
ISA75_RS26820	4533..4763	-	231	WP_100632065.1	hypothetical protein	-
ISA75_RS26825	4768..4974	-	207	WP_148382198.1	hypothetical protein	-
ISA75_RS26830	5008..5424	-	417	WP_016946796.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA75_RS26835	5421..5651	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA75_RS26840	6247..8457	-	2211	WP_032411315.1	AAA family ATPase	-
ISA75_RS26845	8974..9402	+	429	WP_001554382.1	GFA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..303115	303115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.56 Da
• Isoelectric Point: 8.5403

>T179500 WP_016946796.1 NZ_CP063918:c5424-5008 [Klebsiella variicola]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T179500 NZ_CP084803:c4877777-4877493 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGGAGAATCCGCGAATTGCATCGGCCAGTCTGAGTGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGGGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT179500 WP_001261276.1 NZ_CP063918:c5651-5421 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179500 NZ_CP084803:c4878009-4877767 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTGCGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCAGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023