Detailed information of TA system TA179499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4724567..4725157
• Replicon: chromosome
• Accession: NZ_CP063917
• Organism: Klebsiella variicola strain BM374-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ISA75_RS22905
• Protein ID: WP_023322934.1
• Coordinates: 4724567..4724899 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: ISA75_RS22910
• Protein ID: WP_012541132.1
• Coordinates: 4724900..4725157 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA75_RS22880	4720605..4720850	+	246	WP_008804171.1	signal transduction protein PmrD	-
ISA75_RS22885	4720981..4722435	+	1455	WP_008804170.1	AMP nucleosidase	-
ISA75_RS22895	4722808..4724244	-	1437	WP_049153183.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
ISA75_RS22905	4724567..4724899	-	333	WP_023322934.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISA75_RS22910	4724900..4725157	-	258	WP_012541132.1	antitoxin	Antitoxin
ISA75_RS22915	4725685..4726785	+	1101	WP_110218868.1	phosphotransferase	-
ISA75_RS22920	4726782..4727753	-	972	WP_012541130.1	sensor domain-containing diguanylate cyclase	-
ISA75_RS22925	4727960..4728796	-	837	WP_008804155.1	alpha/beta hydrolase	-
ISA75_RS22930	4728974..4729549	-	576	WP_032733307.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11768.58 Da
• Isoelectric Point: 9.2926

>T179499 WP_023322934.1 NZ_CP063917:c4724899-4724567 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPGTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T179499 NZ_CP084803:4337244-4337582 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAAAACTCTTCCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTGCTCGTCTGGCAAACGCTACTTACACG
CCTGCTGGAGCAACACTATGGCCTTACCCTGAATGATACGCCGTTCAGTGATGAGAACGTTATTCAGGAGCATATCAACG
ACGGTATCACGCTGGCGGATGCCATTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGGGGGTTTAAC
TGTCAGGAGCAGTCCCCTTACCTTCGGGCGGTAGATATTTTACGGGCACGACAGGCAACCGGCCTGCTGCGGCAACGCCA
TCATCCTTCGGCACGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT179499 WP_012541132.1 NZ_CP063917:c4725157-4724900 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT179499 NZ_CP084803:4336890-4337219 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGGATAAAGAAACCGACACAGTACGGTGGGGACTCAACCGACCCGGTTCTCCTCAACTTGGTGCACGTCTGGTACAGGA
AGGCAACCGCCTGCATTATTTAGCTGACCGCGCCGGAACAACTGGCGCGTTCAATGAAGAAGAGTGTCACCAGTTGAATA
GCGCATTTCCGCACTTTATAGAGCAGATGGAATTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCACATCGCGTC
ACTCTGTACCACAACGAACTGACCTGTGAAGCGGATACGCTCGGTAGCTGTGGCTACGTTTATATCGCTATCTATCCCAC
TCAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3