Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2343149..2343768 Replicon chromosome
Accession NZ_CP063917
Organism Klebsiella variicola strain BM374-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA75_RS11270 Protein ID WP_002892050.1
Coordinates 2343149..2343367 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA75_RS11275 Protein ID WP_008805436.1
Coordinates 2343394..2343768 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA75_RS11235 2339192..2339455 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA75_RS11240 2339455..2339595 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA75_RS11245 2339592..2340290 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA75_RS11250 2340391..2341848 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
ISA75_RS11255 2341817..2342287 - 471 WP_008805434.1 YlaC family protein -
ISA75_RS11260 2342327..2342452 + 126 Protein_2184 hypothetical protein -
ISA75_RS11265 2342424..2342990 - 567 WP_016160393.1 maltose O-acetyltransferase -
ISA75_RS11270 2343149..2343367 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA75_RS11275 2343394..2343768 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA75_RS11280 2344258..2347404 - 3147 WP_094955339.1 multidrug efflux RND transporter permease subunit AcrB -
ISA75_RS11285 2347427..2348620 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179489 WP_002892050.1 NZ_CP063917:c2343367-2343149 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179489 NZ_CP084783:c1198589-1198371 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179489 WP_008805436.1 NZ_CP063917:c2343768-2343394 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179489 NZ_CP084783:c1198990-1198616 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References