Detailed information of TA system TA179488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1656683..1658403
• Replicon: chromosome
• Accession: NZ_CP063917
• Organism: Klebsiella variicola strain BM374-1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: ISA75_RS08015
• Protein ID: WP_194400832.1
• Coordinates: 1657747..1658403 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: ISA75_RS08010
• Protein ID: WP_194400831.1
• Coordinates: 1656683..1657750 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA75_RS07995	1651914..1652834	+	921	WP_194400825.1	HrgA protein	-
ISA75_RS08000	1652851..1653567	-	717	WP_194400827.1	M48 family metallopeptidase	-
ISA75_RS08005	1653567..1656662	-	3096	WP_194400829.1	HsdR family type I site-specific deoxyribonuclease	-
ISA75_RS08010	1656683..1657750	-	1068	WP_194400831.1	macro domain-containing protein	Antitoxin
ISA75_RS08015	1657747..1658403	-	657	WP_194400832.1	DUF4433 domain-containing protein	Toxin
ISA75_RS08020	1658416..1659705	-	1290	WP_194401002.1	restriction endonuclease subunit S	-
ISA75_RS08025	1659756..1662194	-	2439	WP_194400834.1	type I restriction-modification system subunit M	-
ISA75_RS08030	1662234..1662473	-	240	WP_045330697.1	hypothetical protein	-
ISA75_RS08035	1662822..1663010	+	189	Protein_1555	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25161.65 Da
• Isoelectric Point: 7.2046

>T179488 WP_194400832.1 NZ_CP063917:c1658403-1657747 [Klebsiella variicola]
MAYDYSANLNPEKALIWRIVHRDNIPWILDNGLHCGNSPVQAENWINIGNPELIGKRAGHPVPAGTGGTLHDYVPFYFTP
FSPMLMNIRSGRGGIKQRPNEEIVILVSSLRHVAAQQVPFVFTDSHAYYNLANYYTDLNNLHRIDWPILQARDFRRDQDD
LAKFERYQAEALIWQHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T179488 NZ_CP084783:494908-495189 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40883.11 Da
• Isoelectric Point: 5.7103

>AT179488 WP_194400831.1 NZ_CP063917:c1657750-1656683 [Klebsiella variicola]
MMTYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYALACKQKQVMTGKMFITETSELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKST
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHVNLWLNNEAKEWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGECQPTLDSVKEG
LHQWPAGERWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT179488 NZ_CP084783:494676-494918 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA