Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1122510..1123135 Replicon chromosome
Accession NZ_CP063917
Organism Klebsiella variicola strain BM374-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISA75_RS05515 Protein ID WP_008807903.1
Coordinates 1122752..1123135 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA75_RS05510 Protein ID WP_004150355.1
Coordinates 1122510..1122752 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA75_RS05485 1118500..1119099 + 600 WP_008807908.1 glucose-1-phosphatase -
ISA75_RS05490 1119093..1119953 + 861 WP_008807907.1 virulence factor BrkB family protein -
ISA75_RS05495 1119950..1120387 + 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA75_RS05500 1120432..1121373 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
ISA75_RS05505 1121388..1122305 - 918 WP_110216707.1 alpha/beta hydrolase -
ISA75_RS05510 1122510..1122752 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA75_RS05515 1122752..1123135 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA75_RS05520 1123309..1124238 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA75_RS05525 1124235..1124870 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISA75_RS05530 1124867..1125769 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179487 WP_008807903.1 NZ_CP063917:1122752-1123135 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179487 NZ_CP084780:54983-55465 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179487 WP_004150355.1 NZ_CP063917:1122510-1122752 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179487 NZ_CP084780:54729-54995 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References