Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 725678..726264 Replicon chromosome
Accession NZ_CP063917
Organism Klebsiella variicola strain BM374-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag ISA75_RS03695 Protein ID WP_002920800.1
Coordinates 725678..726046 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A1F2M0C2
Locus tag ISA75_RS03700 Protein ID WP_032731361.1
Coordinates 726043..726264 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA75_RS03675 721162..722232 - 1071 WP_194400711.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
ISA75_RS03680 722234..723079 - 846 WP_044643074.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA75_RS03685 723076..723963 - 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA75_RS03690 724070..725386 - 1317 WP_048268284.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA75_RS03695 725678..726046 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA75_RS03700 726043..726264 - 222 WP_032731361.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA75_RS03705 726428..727141 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA75_RS03710 727159..727926 - 768 WP_194400713.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA75_RS03715 727923..729200 - 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
ISA75_RS03720 729197..730123 - 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 720425..741299 20874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179486 WP_002920800.1 NZ_CP063917:c726046-725678 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179486 NZ_CP084779:c4558817-4558536 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8165.18 Da        Isoelectric Point: 4.7769

>AT179486 WP_032731361.1 NZ_CP063917:c726264-726043 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179486 NZ_CP084779:c4559049-4558807 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M0C2

References