Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 255946..256603 Replicon chromosome
Accession NZ_CP063917
Organism Klebsiella variicola strain BM374-1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISA75_RS01265 Protein ID WP_002916310.1
Coordinates 255946..256356 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISA75_RS01270 Protein ID WP_002916312.1
Coordinates 256337..256603 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA75_RS01245 251946..253679 - 1734 WP_049139238.1 single-stranded-DNA-specific exonuclease RecJ -
ISA75_RS01250 253685..254398 - 714 WP_008806425.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISA75_RS01255 254421..255317 - 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
ISA75_RS01260 255418..255939 + 522 WP_086070691.1 flavodoxin FldB -
ISA75_RS01265 255946..256356 - 411 WP_002916310.1 protein YgfX Toxin
ISA75_RS01270 256337..256603 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISA75_RS01275 256849..257832 + 984 WP_042947382.1 tRNA-modifying protein YgfZ -
ISA75_RS01280 258092..258751 - 660 WP_008806429.1 hemolysin III family protein -
ISA75_RS01285 258915..259226 - 312 WP_194400676.1 N(4)-acetylcytidine aminohydrolase -
ISA75_RS01290 259277..260005 + 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
ISA75_RS01295 260125..261558 + 1434 WP_046881934.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179484 WP_002916310.1 NZ_CP063917:c256356-255946 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179484 NZ_CP084779:3846256-3846474 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179484 WP_002916312.1 NZ_CP063917:c256603-256337 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179484 NZ_CP084779:3845855-3846229 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References