Detailed information of TA system TA179474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3719719..3721307
• Replicon: chromosome
• Accession: NZ_CP063916
• Organism: Klebsiella variicola strain BS327-2-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ISA76_RS17985
• Protein ID: WP_194387132.1
• Coordinates: 3719985..3721307 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISA76_RS17980
• Protein ID: WP_008804920.1
• Coordinates: 3719719..3719985 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA76_RS17955	3714869..3715792	+	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA76_RS17960	3715773..3716927	-	1155	WP_194387134.1	FAD-dependent urate hydroxylase HpxO	-
ISA76_RS17965	3717273..3718652	+	1380	WP_043875298.1	purine permease	-
ISA76_RS17970	3718630..3719130	+	501	WP_023297604.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA76_RS17975	3719127..3719453	+	327	WP_194387133.1	hydroxyisourate hydrolase	-
ISA76_RS17980	3719719..3719985	+	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA76_RS17985	3719985..3721307	+	1323	WP_194387132.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA76_RS17990	3721324..3722616	-	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISA76_RS17995	3722749..3723753	-	1005	WP_039103406.1	type 1 fimbrial protein	-
ISA76_RS18000	3723741..3726242	-	2502	WP_110196451.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48969.34 Da
• Isoelectric Point: 7.9109

>T179474 WP_194387132.1 NZ_CP063916:3719985-3721307 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADTVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESVRRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179474 NZ_CP084777:789012-789422 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTTGGGACGCCATGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179474 WP_008804920.1 NZ_CP063916:3719719-3719985 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179474 NZ_CP084777:788765-789031 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGACATTAACAATAAAGCTCGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MCN9