Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 686319..686944 Replicon chromosome
Accession NZ_CP063916
Organism Klebsiella variicola strain BS327-2-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag ISA76_RS03445 Protein ID WP_008807903.1
Coordinates 686319..686702 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA76_RS03450 Protein ID WP_004150355.1
Coordinates 686702..686944 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA76_RS03430 683685..684587 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ISA76_RS03435 684584..685219 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
ISA76_RS03440 685216..686145 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA76_RS03445 686319..686702 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA76_RS03450 686702..686944 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA76_RS03455 687149..688066 + 918 WP_043875979.1 alpha/beta hydrolase -
ISA76_RS03460 688081..689022 - 942 WP_022064599.1 fatty acid biosynthesis protein FabY -
ISA76_RS03465 689067..689504 - 438 WP_043875980.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA76_RS03470 689501..690361 - 861 WP_008807907.1 virulence factor BrkB family protein -
ISA76_RS03475 690355..690954 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179468 WP_008807903.1 NZ_CP063916:c686702-686319 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T179468 NZ_CP084770:c4650974-4650693 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179468 WP_004150355.1 NZ_CP063916:c686944-686702 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179468 NZ_CP084770:c4651206-4650964 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References