Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 95660..96317 Replicon chromosome
Accession NZ_CP063916
Organism Klebsiella variicola strain BS327-2-1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISA76_RS00490 Protein ID WP_002916310.1
Coordinates 95660..96070 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISA76_RS00495 Protein ID WP_002916312.1
Coordinates 96051..96317 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA76_RS00470 91660..93393 - 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -
ISA76_RS00475 93399..94112 - 714 WP_194386987.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISA76_RS00480 94135..95031 - 897 WP_194386986.1 site-specific tyrosine recombinase XerD -
ISA76_RS00485 95132..95653 + 522 WP_008806427.1 flavodoxin FldB -
ISA76_RS00490 95660..96070 - 411 WP_002916310.1 protein YgfX Toxin
ISA76_RS00495 96051..96317 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISA76_RS00500 96563..97546 + 984 WP_043876073.1 tRNA-modifying protein YgfZ -
ISA76_RS00505 97593..98000 - 408 WP_131023732.1 hypothetical protein -
ISA76_RS00510 98066..98725 - 660 WP_043876072.1 hemolysin III family protein -
ISA76_RS00515 98889..99200 - 312 WP_012540591.1 N(4)-acetylcytidine aminohydrolase -
ISA76_RS00520 99251..99979 + 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179467 WP_002916310.1 NZ_CP063916:c96070-95660 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179467 NZ_CP084770:3962144-3962362 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179467 WP_002916312.1 NZ_CP063916:c96317-96051 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179467 NZ_CP084770:3961743-3962117 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References