Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5221158..5221789 Replicon chromosome
Accession NZ_CP063915
Organism Klebsiella variicola strain M186-1-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag ISA77_RS25215 Protein ID WP_044650325.1
Coordinates 5221514..5221789 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag ISA77_RS25210 Protein ID WP_004181489.1
Coordinates 5221158..5221517 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA77_RS25185 5217415..5218359 + 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -
ISA77_RS25190 5218356..5219189 + 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
ISA77_RS25195 5219189..5219953 + 765 WP_008806946.1 nickel import ATP-binding protein NikD -
ISA77_RS25200 5219950..5220741 + 792 WP_008806945.1 nickel import ATP-binding protein NikE -
ISA77_RS25205 5220729..5221130 + 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
ISA77_RS25210 5221158..5221517 - 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISA77_RS25215 5221514..5221789 - 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
ISA77_RS25220 5222186..5223199 + 1014 WP_022066424.1 magnesium transporter -
ISA77_RS25225 5223239..5224432 - 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
ISA77_RS25230 5224662..5226158 + 1497 WP_008806938.1 inorganic phosphate transporter PitA -
ISA77_RS25235 5226218..5226553 - 336 WP_002921203.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T179466 WP_044650325.1 NZ_CP063915:c5221789-5221514 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T179466 NZ_CP084770:c2588304-2586982 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATTACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATTTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCGGACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
TAAACTGCACGCCCGCCTGCAGCAAGGGCTGCTGGCGGGATAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT179466 WP_004181489.1 NZ_CP063915:c5221517-5221158 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT179466 NZ_CP084770:c2588570-2588304 [Klebsiella quasipneumoniae subsp. similipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References