Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4788381..4789156 Replicon chromosome
Accession NZ_CP063915
Organism Klebsiella variicola strain M186-1-2

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag ISA77_RS22970 Protein ID WP_032740995.1
Coordinates 4788381..4788866 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISA77_RS22975 Protein ID WP_004150912.1
Coordinates 4788863..4789156 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA77_RS22950 4785544..4786257 + 714 WP_008806478.1 DUF554 domain-containing protein -
ISA77_RS22960 4786772..4786988 - 217 Protein_4483 transposase -
ISA77_RS22965 4787085..4787677 - 593 Protein_4484 hypothetical protein -
ISA77_RS22970 4788381..4788866 - 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
ISA77_RS22975 4788863..4789156 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISA77_RS22980 4789752..4791128 + 1377 WP_162542795.1 cystathionine beta-synthase -
ISA77_RS22985 4791141..4792289 + 1149 WP_008806488.1 PLP-dependent aspartate aminotransferase family protein -
ISA77_RS22990 4792290..4793201 - 912 WP_023298220.1 LysR family transcriptional regulator -
ISA77_RS22995 4793299..4793901 + 603 WP_012540553.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4786836..4786988 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T179465 WP_032740995.1 NZ_CP063915:c4788866-4788381 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

>T179465 NZ_CP084770:1921982-1922084 [Klebsiella quasipneumoniae subsp. similipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179465 WP_004150912.1 NZ_CP063915:c4789156-4788863 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179465 NZ_CP084770:c1922088-1921941 [Klebsiella quasipneumoniae subsp. similipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References