Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3632948..3633538 Replicon chromosome
Accession NZ_CP063915
Organism Klebsiella variicola strain M186-1-2

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA77_RS17415 Protein ID WP_008804165.1
Coordinates 3632948..3633280 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA77_RS17420 Protein ID WP_012541132.1
Coordinates 3633281..3633538 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA77_RS17390 3628986..3629231 + 246 WP_008804171.1 signal transduction protein PmrD -
ISA77_RS17395 3629362..3630816 + 1455 WP_008804170.1 AMP nucleosidase -
ISA77_RS17405 3631189..3632625 - 1437 WP_162494461.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA77_RS17415 3632948..3633280 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA77_RS17420 3633281..3633538 - 258 WP_012541132.1 antitoxin Antitoxin
ISA77_RS17425 3634067..3635167 + 1101 WP_048268588.1 phosphotransferase -
ISA77_RS17430 3635164..3636135 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA77_RS17435 3636341..3637177 - 837 WP_008804155.1 alpha/beta hydrolase -
ISA77_RS17440 3637354..3637929 - 576 WP_077139353.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179463 WP_008804165.1 NZ_CP063915:c3633280-3632948 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179463 NZ_CP084767:c5463196-5462813 [Klebsiella variicola subsp. tropica]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179463 WP_012541132.1 NZ_CP063915:c3633538-3633281 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179463 NZ_CP084767:c5463438-5463196 [Klebsiella variicola subsp. tropica]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTTGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References