Detailed information of TA system TA179454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2739751..2741339
• Replicon: chromosome
• Accession: NZ_CP063915
• Organism: Klebsiella variicola strain M186-1-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0B7G636
• Locus tag: ISA77_RS12985
• Protein ID: WP_012968244.1
• Coordinates: 2740017..2741339 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISA77_RS12980
• Protein ID: WP_008804920.1
• Coordinates: 2739751..2740017 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA77_RS12955	2734902..2735825	+	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA77_RS12960	2735806..2736960	-	1155	WP_008804924.1	FAD-dependent urate hydroxylase HpxO	-
ISA77_RS12965	2737305..2738684	+	1380	WP_012968246.1	purine permease	-
ISA77_RS12970	2738662..2739162	+	501	WP_023297604.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA77_RS12975	2739159..2739485	+	327	WP_008804921.1	hydroxyisourate hydrolase	-
ISA77_RS12980	2739751..2740017	+	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA77_RS12985	2740017..2741339	+	1323	WP_012968244.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA77_RS12990	2741356..2742648	-	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISA77_RS12995	2742781..2743785	-	1005	WP_032730346.1	type 1 fimbrial protein	-
ISA77_RS13000	2743773..2746274	-	2502	WP_179153723.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48896.29 Da
• Isoelectric Point: 7.9109

>T179454 WP_012968244.1 NZ_CP063915:2740017-2741339 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGELMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179454 NZ_CP084767:362445-362792 [Klebsiella variicola subsp. tropica]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAATTTGGTCCGCAGCTTGGGTGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTTCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCATTTGATCCTCTGCGGAAAGCTATC
ATTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGATAGAGAATT
CAGCAAGCACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179454 WP_008804920.1 NZ_CP063915:2739751-2740017 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179454 NZ_CP084767:362792-363091 [Klebsiella variicola subsp. tropica]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAATCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCATTAAACTTGCTTCGTGAAGAGTTACAAATTTCACAAACGGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGT
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0B7G636

Antitoxin

• AlphaFold DB: A0A1F2MCN9