Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1314753..1315372 Replicon chromosome
Accession NZ_CP063915
Organism Klebsiella variicola strain M186-1-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA77_RS06195 Protein ID WP_002892050.1
Coordinates 1314753..1314971 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA77_RS06200 Protein ID WP_008805436.1
Coordinates 1314998..1315372 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA77_RS06160 1310796..1311059 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA77_RS06165 1311059..1311199 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA77_RS06170 1311196..1311894 - 699 WP_012968815.1 GNAT family N-acetyltransferase -
ISA77_RS06175 1311995..1313452 + 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
ISA77_RS06180 1313421..1313891 - 471 WP_008805434.1 YlaC family protein -
ISA77_RS06185 1313928..1314056 + 129 Protein_1187 hypothetical protein -
ISA77_RS06190 1314028..1314594 - 567 WP_032438710.1 maltose O-acetyltransferase -
ISA77_RS06195 1314753..1314971 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA77_RS06200 1314998..1315372 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA77_RS06205 1315855..1319001 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA77_RS06210 1319024..1320217 - 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179452 WP_002892050.1 NZ_CP063915:c1314971-1314753 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179452 NZ_CP084766:c5390147-5389764 [Klebsiella variicola subsp. variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179452 WP_008805436.1 NZ_CP063915:c1315372-1314998 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179452 NZ_CP084766:c5390389-5390147 [Klebsiella variicola subsp. variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References