Detailed information of TA system TA179449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 249236..249972
• Replicon: plasmid pBM336-2-1
• Accession: NZ_CP063914
• Organism: Klebsiella pneumoniae strain BM336-2-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: ISA78_RS27530
• Protein ID: WP_004187044.1
• Coordinates: 249490..249972 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: ISA78_RS27525
• Protein ID: WP_003026799.1
• Coordinates: 249236..249502 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA78_RS27510	244708..246102	+	1395	WP_032439652.1	cytosine permease	-
ISA78_RS27515	246114..247322	+	1209	WP_032448288.1	imidazolonepropionase	-
ISA78_RS27520	247315..248124	+	810	WP_023329017.1	N-formylglutamate deformylase	-
ISA78_RS27525	249236..249502	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
ISA78_RS27530	249490..249972	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
ISA78_RS27535	250179..251525	+	1347	WP_077254728.1	ISNCY family transposase	-
ISA78_RS27540	251574..251972	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
ISA78_RS27545	252120..252227	-	108	Protein_268	IS3 family transposase	-
ISA78_RS27550	252225..252395	-	171	Protein_269	transposase	-
ISA78_RS27555	252505..253441	+	937	Protein_270	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B)	iucA / iucB / iucC / iucD / iutA	1..256775	256775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T179449 WP_004187044.1 NZ_CP063914:249490-249972 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T179449 NZ_CP084766:4177419-4177637 [Klebsiella variicola subsp. variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT179449 WP_003026799.1 NZ_CP063914:249236-249502 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT179449 NZ_CP084766:4177018-4177392 [Klebsiella variicola subsp. variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66