Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1468047..1468857 Replicon chromosome
Accession NZ_CP063913
Organism Klebsiella pneumoniae strain BM336-2-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISA78_RS06965 Protein ID WP_004178461.1
Coordinates 1468047..1468580 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA78_RS06970 Protein ID WP_002887278.1
Coordinates 1468591..1468857 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA78_RS06960 1466878..1467999 + 1122 WP_020804439.1 cupin domain-containing protein -
ISA78_RS06965 1468047..1468580 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
ISA78_RS06970 1468591..1468857 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA78_RS06975 1468960..1470393 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA78_RS06980 1470383..1471066 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
ISA78_RS06985 1471238..1472623 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
ISA78_RS06990 1472641..1472985 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179432 WP_004178461.1 NZ_CP063913:c1468580-1468047 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179432 NZ_CP084765:329041-329409 [Klebsiella variicola subsp. variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAACCCGGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179432 WP_002887278.1 NZ_CP063913:c1468857-1468591 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179432 NZ_CP084765:328823-329044 [Klebsiella variicola subsp. variicola]
ATGAAAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAATTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References