Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 880086..880705 Replicon chromosome
Accession NZ_CP063913
Organism Klebsiella pneumoniae strain BM336-2-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA78_RS04155 Protein ID WP_002892050.1
Coordinates 880487..880705 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA78_RS04150 Protein ID WP_002892066.1
Coordinates 880086..880460 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA78_RS04140 875238..876431 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
ISA78_RS04145 876454..879600 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA78_RS04150 880086..880460 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA78_RS04155 880487..880705 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA78_RS04160 880864..881430 + 567 WP_002892030.1 maltose O-acetyltransferase -
ISA78_RS04165 881402..881542 - 141 WP_004147370.1 hypothetical protein -
ISA78_RS04170 881563..882033 + 471 WP_002892026.1 YlaC family protein -
ISA78_RS04175 882008..883459 - 1452 WP_004177237.1 PLP-dependent aminotransferase family protein -
ISA78_RS04180 883560..884258 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
ISA78_RS04185 884255..884395 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA78_RS04190 884395..884658 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179431 WP_002892050.1 NZ_CP063913:880487-880705 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179431 NZ_CP084765:287521-287796 [Klebsiella variicola subsp. variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179431 WP_002892066.1 NZ_CP063913:880086-880460 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179431 NZ_CP084765:287793-288152 [Klebsiella variicola subsp. variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References