Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 68515..69146 Replicon chromosome
Accession NZ_CP063913
Organism Klebsiella pneumoniae strain BM336-2-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag ISA78_RS00415 Protein ID WP_012542177.1
Coordinates 68970..69146 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X1QFN9
Locus tag ISA78_RS00410 Protein ID WP_012542176.1
Coordinates 68515..68922 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA78_RS00370 65143..65577 - 435 WP_012542168.1 phage terminase small subunit P27 family -
ISA78_RS00375 65827..66258 - 432 WP_077255824.1 hypothetical protein -
ISA78_RS00380 66255..66572 - 318 WP_020803188.1 hypothetical protein -
ISA78_RS00385 66524..66885 - 362 Protein_76 HNH endonuclease -
ISA78_RS00390 66869..67075 - 207 WP_032447250.1 hypothetical protein -
ISA78_RS00395 67111..67584 - 474 WP_194429174.1 DUF2514 domain-containing protein -
ISA78_RS00400 67617..68147 - 531 WP_023328100.1 lysozyme -
ISA78_RS00405 68125..68361 - 237 WP_004111739.1 class II holin family protein -
ISA78_RS00410 68515..68922 - 408 WP_012542176.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ISA78_RS00415 68970..69146 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
ISA78_RS00420 69509..70669 + 1161 WP_194429127.1 DUF262 domain-containing protein -
ISA78_RS00425 70666..71355 + 690 WP_012542179.1 hypothetical protein -
ISA78_RS00430 71321..72355 - 1035 Protein_85 DNA cytosine methyltransferase -
ISA78_RS00435 72445..72789 - 345 WP_032447265.1 antitermination protein -
ISA78_RS00440 72802..73828 - 1027 Protein_87 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1314..86882 85568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T179430 WP_012542177.1 NZ_CP063913:c69146-68970 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T179430 NZ_CP084760:6094-6354 [Shinella sp. XGS7]
ATGGAATTAAGCAGGAAGCATCAGGCAACTCTTGAAGCCGTCTTTGCCGAACCCGTCCGCGCCAGCATACCTTGGCGCGA
CATCGAGGCCATGTTGGCCGCATGTGGGGCGGAGATTACGGAAGGGCAAGGGTCGCGTGTCCGCATAGCCTTGAATGGCG
TGCGGGCGGTGTTTCACCGACCCCATCCGCAAAAGGAAACGGACAAGGGCGCGGTGAAATCCGTCCGGCGCTTCCTGCTC
GAAGCGGAGATACAACCATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14977.99 Da        Isoelectric Point: 4.4277

>AT179430 WP_012542176.1 NZ_CP063913:c68922-68515 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT179430 NZ_CP084760:6351-6680 [Shinella sp. XGS7]
ATGACCATGATGCACTACAAGGGCTATGAAGCTGTCGTCGAGTTCGACGAGGAGGCCGAGATTTTCCATGGCGAAGTCAT
CAACCTGCGCGATGTGATTACCTTCCAGGGCGCATCGGCGGCCGAGCTGAAGCAAGCATTTGCGGAATCTGTCGAGGATT
ACCTGGCATTCTGCGCCGAGCGCGGCGAGGAACCGGAAAAGCCCTTCTCCGGACAGTTCGTGGTGCGTACCGAACCAGGC
TTGCATAAAGCTCTGACGGTGGCCGCCCGCCGTGCCGGCGTCAGTCTGAACAAGTGGGTTACGTCCACGTTGGAACGAGC
TGTCGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1QFN9

References