Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4851727..4852317 Replicon chromosome
Accession NZ_CP063912
Organism Klebsiella variicola strain BS359-2-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA79_RS23350 Protein ID WP_008804165.1
Coordinates 4851985..4852317 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA79_RS23345 Protein ID WP_012541132.1
Coordinates 4851727..4851984 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA79_RS23325 4847333..4847908 + 576 WP_047666760.1 hypothetical protein -
ISA79_RS23330 4848087..4848923 + 837 WP_008804155.1 alpha/beta hydrolase -
ISA79_RS23335 4849129..4850100 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA79_RS23340 4850097..4851197 - 1101 WP_032754894.1 phosphotransferase -
ISA79_RS23345 4851727..4851984 + 258 WP_012541132.1 antitoxin Antitoxin
ISA79_RS23350 4851985..4852317 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA79_RS23360 4852640..4854076 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA79_RS23370 4854449..4855903 - 1455 WP_008804170.1 AMP nucleosidase -
ISA79_RS23375 4856034..4856279 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179420 WP_008804165.1 NZ_CP063912:4851985-4852317 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179420 NZ_CP084742:c6624614-6624264 [Pseudomonas sp. L5B5]
ATGAATGCTCTATTTATTGAACTACCGGCATTTGGGCGATACCGAAGGGACTTCCTTGATGACGAGTTGTTCTGCGAATT
GCAGATCGAGTTACTGCGCCACCCGCACGCAGGTGTCTTCATCCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCATCG
ATGAGCGGCGCAACAAGGGCCGACGTGGCGGGCTGAGGGTAATTTATTACTGGTGGTCCGGAGGGGCGCAGTTCTGGCTC
TTTACCTTGTTCGACAAGGACCAGTTGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAATTGCTGGAACGTGA
AATCGAGGCCAGAAGACATGACCAAGCGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179420 WP_012541132.1 NZ_CP063912:4851727-4851984 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179420 NZ_CP084742:c6624277-6623960 [Pseudomonas sp. L5B5]
ATGACCAAGCGTGACATCTTTTCCGAACTGCTGGAGGGCATCGACGCCCTCGCCCAGGAACGCCAGGGCAAGATCACCCT
GCGCAGCCATAACGTGAAACTCGACAAGCTACCACCCATCACCGCCGCCGAGGTGATGAATATCCGCCAGCAACTCAACC
TCTCGCGCTCGGTATTCGCCATGTACCTACGCACCAACACCCGCACCCTGGAGAACTGGGAACAAGGCCGAGCTACCCCC
AATGCCCAGGCCACTACCCTGATGCGCCTGGTGGAGCGCTATCCCGACACGGTGGAACGCCTCGCTTCGCTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References