Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3832672..3833329 Replicon chromosome
Accession NZ_CP063912
Organism Klebsiella variicola strain BS359-2-1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISA79_RS18540 Protein ID WP_002916310.1
Coordinates 3832919..3833329 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISA79_RS18535 Protein ID WP_002916312.1
Coordinates 3832672..3832938 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA79_RS18510 3829010..3829738 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
ISA79_RS18515 3829789..3830100 + 312 WP_012540591.1 N(4)-acetylcytidine aminohydrolase -
ISA79_RS18520 3830264..3830923 + 660 WP_043876072.1 hemolysin III family protein -
ISA79_RS18525 3831058..3831396 + 339 WP_194386985.1 hypothetical protein -
ISA79_RS18530 3831443..3832426 - 984 WP_043876073.1 tRNA-modifying protein YgfZ -
ISA79_RS18535 3832672..3832938 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISA79_RS18540 3832919..3833329 + 411 WP_002916310.1 protein YgfX Toxin
ISA79_RS18545 3833336..3833857 - 522 WP_008806427.1 flavodoxin FldB -
ISA79_RS18550 3833958..3834854 + 897 WP_043876074.1 site-specific tyrosine recombinase XerD -
ISA79_RS18555 3834877..3835590 + 714 WP_012540593.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISA79_RS18560 3835596..3837329 + 1734 WP_043876075.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179419 WP_002916310.1 NZ_CP063912:3832919-3833329 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179419 NZ_CP084742:1559536-1559718 [Pseudomonas sp. L5B5]
GTGCAAAGCAGACAGCTAATCAAGGAGCTTGAAGCCGCTGGATGGATACTGGACCGGATAACCGGAAGCCACCATCTGTT
CAAGCATCCTTACAGACCCAACACCGTTCCGGTGCCTCACCCGAAGAAGGACCTGCCACGGGGAACGGTCAGGGCCATCC
GGAAACTGGCCGAACTGATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179419 WP_002916312.1 NZ_CP063912:3832672-3832938 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179419 NZ_CP084742:1559754-1560155 [Pseudomonas sp. L5B5]
ATGCAATATCCCATCTGCATCGAATGGGGCGACGAACACACCGCCACCGGCATCCAGATTCCCGACATCCCCGGTGCAGT
CACCGCCGGCGACACCTTCGAGAGCGCCTACAGCGCCGCCATCGAGATCGCCCACGTCATGCTCGAAGAACTGGCCCGCG
CCGGGCAAGCCATTCCCCTGCCCTCGCCCACCCACAAGCACCGCGCCAACCCCGATTTCACAGGCATGGGCTGGGGCATG
CTGGACATCGACATCACGCCCTACCTGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCCGGTTATGTGATTCAGCAGAT
CGACCGCTTTGTCCGCGAGCACAACATCAAGAGCCGGTCGTCCTTCCTGGCCGATGCCGCGCTGGAAAAGCTCGGGCGCT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References