Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3349406..3349992 Replicon chromosome
Accession NZ_CP063912
Organism Klebsiella variicola strain BS359-2-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag ISA79_RS16005 Protein ID WP_002920800.1
Coordinates 3349624..3349992 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A1F2M0C2
Locus tag ISA79_RS16000 Protein ID WP_032731361.1
Coordinates 3349406..3349627 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA79_RS15980 3345563..3346489 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ISA79_RS15985 3346486..3347763 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
ISA79_RS15990 3347760..3348527 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ISA79_RS15995 3348529..3349242 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ISA79_RS16000 3349406..3349627 + 222 WP_032731361.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISA79_RS16005 3349624..3349992 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ISA79_RS16010 3350284..3351600 + 1317 WP_008806974.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ISA79_RS16015 3351707..3352594 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ISA79_RS16020 3352591..3353436 + 846 WP_032740290.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ISA79_RS16025 3353438..3354508 + 1071 WP_023323353.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3334512..3355245 20733


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T179417 WP_002920800.1 NZ_CP063912:3349624-3349992 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T179417 NZ_CP084741:4891066-4892385 [Pectobacterium carotovorum]
ATGGCTGCGCTCGATGTTTACATGAACGGCTATCGGGTGGGGATTCTGACCAAAACGGGCAGTGGGGCACATCATTTTTC
CTATGATGCGAACTGGCTCGGATTGCCGGGGAGTCGCCCCATTTCGCTTTCTATGCCGCTACGTCATCAGCCCTATCAAG
GCGATGAAGTTTATAATTTCTTCGACAATTTGTTGCCGGACAACCCGGATATCAGAAGACGGATTGTCGCCAGGCATCAC
GCGGATTCCACCCAACCGTTTGATCTGCTGGATAAAGTGGGGAAAGACAGTGTCGGGGCGTTACAATTAGTGCCGCAGGG
TACACCTGTGCACGATATAAAAAAGATCGAATACAAAGCCTTATCCGAGCAGCAGTTTGAAAACATACTGACGGGATATC
TGTACGATGCCCCGCTGGGGATGATTGATACTGAAGACGATTTTCGTATTTCTATCGCAGGTGCACAGGAAAAGACGGCG
CTGCTTTATCTGGATGATCACTGGTGCCTGCCTCTCAATGCCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATCGAGAGTCACTCTTACTCAATTGATATGTCTGACAGCGTTGAAAATGAGTATCTGTGCCTGCTCATTGCCAATGCAT
TTGGGCTGCCAGTGCCGCACTGTTTTATGATCAACGCTGGCAAGATAAAGGTATTGGCGGTGGAGCGTTTCGATCGTAAA
TACGCCTCGGATGTCAGTTGGATTATGCGGCTACCACAAGAGGATTTTTGTCAGGTATTAAACGTTCCTTCCGCACTGAA
ATATGAAAATCATGGTGGACCGGGTATTTCCGCCATTATGTCGTTTCTATTGGGTTCGGTTGATCCAGAAAGAGATCGTT
ATCGCTTTATGCAGGCTCAGGTACTGTTTTGGCTATTGGCTGCAACGGATGGTCATGCGAAAAACTTTTCCCTGTTTATT
GAATCTGAGGGGCGATATCGACTCACACCTTTCTATGACATTCTCTCTATGTATCCGGCGATGGGCGGACGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGATTGACTGGCTCAAGAGGCAAGAAATATGCGATTGAGCAGATCTTTCCCCGAC
ATTTTTTCCAGACGGCGAAGGCCGTTGGGTTTGCCAGAGAATCGATGGAAAACATTCTGGTGGAGTTTTCAAACACCGTT
GAGACCGTCATGACGACTGTTAGAAGCCAGCTACCTGTGGATTTTCCTACTCATATCAGTGAGGCAATTCTGGCCGGATT
GCAGGCCAGATCGAGACGGCTCATGAGCGGATGGGAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8165.18 Da        Isoelectric Point: 4.7769

>AT179417 WP_032731361.1 NZ_CP063912:3349406-3349627 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGIPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT179417 NZ_CP084741:4890797-4891066 [Pectobacterium carotovorum]
ATGATGAAGATCACCAACAGCAAGCAGCTTAGCAGCTATCTGAAAGATGTCCGTATCACGCAGAAGCTTTCACAAGGGAA
AGTAGCCAGTAAAGTGGGTATTCGCCAGGATACGGTATCCAGTTTCGAGCAACATCCTGATTCCACTAAGCTGGAAACCT
TCTTTAAAATCCTGTCGGCATTGAATTTAGAACTCACTGTTACGCCCAGGAATGTGGACACCACCAATAACGAAGCCTCT
GTTGCATCATCCTGGAAGGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M0C2

References