Detailed information of TA system TA179412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 221270..222858
• Replicon: chromosome
• Accession: NZ_CP063912
• Organism: Klebsiella variicola strain BS359-2-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0B7G636
• Locus tag: ISA79_RS01095
• Protein ID: WP_012968244.1
• Coordinates: 221270..222592 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISA79_RS01100
• Protein ID: WP_008804920.1
• Coordinates: 222592..222858 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA79_RS01080	216335..218836	+	2502	WP_043875295.1	fimbrial biogenesis outer membrane usher protein	-
ISA79_RS01085	218824..219828	+	1005	WP_032730346.1	type 1 fimbrial protein	-
ISA79_RS01090	219961..221253	+	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISA79_RS01095	221270..222592	-	1323	WP_012968244.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA79_RS01100	222592..222858	-	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA79_RS01105	223124..223450	-	327	WP_008804921.1	hydroxyisourate hydrolase	-
ISA79_RS01110	223447..223947	-	501	WP_008804922.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA79_RS01115	223925..225304	-	1380	WP_043875298.1	purine permease	-
ISA79_RS01120	225650..226804	+	1155	WP_008804924.1	FAD-dependent urate hydroxylase HpxO	-
ISA79_RS01125	226785..227708	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48896.29 Da
• Isoelectric Point: 7.9109

>T179412 WP_012968244.1 NZ_CP063912:c222592-221270 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGELMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179412 NZ_CP084741:c1291677-1291474 [Pectobacterium carotovorum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCTGAACTGACAATGAACAAAC
TCTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179412 WP_008804920.1 NZ_CP063912:c222858-222592 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179412 NZ_CP084741:c1292099-1291731 [Pectobacterium carotovorum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGTCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGAATTAATGATGCTGAACTACGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7G636

Antitoxin

• AlphaFold DB: A0A1F2MCN9