Detailed information of TA system TA179409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3194327..3196008
• Replicon: chromosome
• Accession: NZ_CP063911
• Organism: Klebsiella variicola strain M186-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: ISA80_RS15535
• Protein ID: WP_022065798.1
• Coordinates: 3195091..3196008 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A0B7GCX3
• Locus tag: ISA80_RS15530
• Protein ID: WP_008807257.1
• Coordinates: 3194327..3195085 (+)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA80_RS15515	3189790..3190476	-	687	WP_008807260.1	sel1 repeat family protein	-
ISA80_RS15520	3191094..3193241	-	2148	WP_077625434.1	formate dehydrogenase subunit alpha	-
ISA80_RS15525	3193514..3194242	+	729	WP_008807258.1	response regulator transcription factor	-
ISA80_RS15530	3194327..3195085	+	759	WP_008807257.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
ISA80_RS15535	3195091..3196008	+	918	WP_022065798.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA80_RS15540	3196133..3197140	-	1008	WP_012969142.1	carbohydrate kinase family protein	-
ISA80_RS15545	3197215..3198156	-	942	WP_012543155.1	ABC transporter substrate-binding protein	-
ISA80_RS15550	3198187..3199185	-	999	WP_023296904.1	ribose ABC transporter permease	-
ISA80_RS15555	3199182..3200702	-	1521	WP_008807253.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34751.14 Da
• Isoelectric Point: 5.4707

>T179409 WP_022065798.1 NZ_CP063911:3195091-3196008 [Klebsiella variicola]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRTIALPHIRDALARIAANLQQQ
AGMNAVLQANRSDEMRVVVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELNRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPYRMIDALKS
CFTQQDIDFLLSFKRGEPDWRLAPETRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T179409 NZ_CP084741:c857125-856874 [Pectobacterium carotovorum]
ATGAGCTATAAGCTGGGGTTTGAAGAGCACGCGCTGAAAGAATTTAAGAAGTTGAGCGCTCCGATACGGGAGCAGTTCAC
CAAAAAACTGAAAGAAATTTTACAAAATCCCCACGTTCCCGCCAATCGTCTTCACGGAATGGCTGACTGCTACAAGAAGT
TTATAAAGCAGCCAAAGACCGACTATAAGCGCGGACAGTTTTCAACTCGCGGCTATTTTGTTGTGCAACCTATCTATCAA
GGTAATGAGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28653.01 Da
• Isoelectric Point: 10.1988

>AT179409 WP_008807257.1 NZ_CP063911:3194327-3195085 [Klebsiella variicola]
MPSKLNWLLQNSAPGDVILQSWLSRHAISPSLAFKYTQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSMENKALLPKWFREFPGVEWIVISGQKLPVLDDKYRVTLDVKGKRLTGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQTKRLYLFFASFYEHGWLRRIDSQKIDLGAGKRQIVVNGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT179409 NZ_CP084741:c857366-857115 [Pectobacterium carotovorum]
ATGTCCGTACAACCTATCCTTGCAAATGCAGCTGTGAGCTGCAGCGACTTTAAAAAATCGCCCAATGCCGCGCTTAAAGA
AGCTAACGGTGAGCCTGTAGCGGTGCTCACTAATGGCCGTATCTCTGGCTACTATGTTTCCCCGGAAACATGGGAGGCGC
TGGCCGACTATCAGGAAGATATCGAACTTGCCAAAATCGCCCGCTCACAGATGAAGGGGATGCGTGTGAAGGTCGATCTG
GATGAGCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B7GCX3