Detailed information of TA system TA179408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2872211..2872836
• Replicon: chromosome
• Accession: NZ_CP063911
• Organism: Klebsiella variicola strain M186-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1F2M041
• Locus tag: ISA80_RS14045
• Protein ID: WP_008807903.1
• Coordinates: 2872453..2872836 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ISA80_RS14040
• Protein ID: WP_162550505.1
• Coordinates: 2872211..2872453 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA80_RS14015	2868202..2868801	+	600	WP_008807908.1	glucose-1-phosphatase	-
ISA80_RS14020	2868795..2869655	+	861	WP_008807907.1	virulence factor BrkB family protein	-
ISA80_RS14025	2869652..2870089	+	438	WP_012543288.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ISA80_RS14030	2870134..2871075	+	942	WP_012543287.1	fatty acid biosynthesis protein FabY	-
ISA80_RS14035	2871090..2872007	-	918	WP_060876598.1	alpha/beta hydrolase	-
ISA80_RS14040	2872211..2872453	+	243	WP_162550505.1	ribbon-helix-helix domain-containing protein	Antitoxin
ISA80_RS14045	2872453..2872836	+	384	WP_008807903.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA80_RS14050	2873010..2873939	-	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
ISA80_RS14055	2873936..2874571	-	636	WP_008807902.1	formate dehydrogenase cytochrome b556 subunit	-
ISA80_RS14060	2874568..2875470	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T179408 WP_008807903.1 NZ_CP063911:2872453-2872836 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Nucleotide

• Length: 384 bp

>T179408 NZ_CP084741:768731-769144 [Pectobacterium carotovorum]
GTGGCCCAGTGGCGATGTGACCTTCGTGTTTCCTGGCGCATGCAACTGCTGTCATTGGTTGTGCATGGCCTATTAGTATT
GCTCATTCTGCTGGCACCGTGGCCGGACGGCTACGCGTGGCTATGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAACGGAATATTAAATCCCGACAGGGGGAAATCGTTCTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGCTGCTGAAGAATGGCGTATTACTGTCATTACAAGCGGTTAACGGTAAAGACAA
GCAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGACGAATGGCGTCACTTGCGTCAAATTCTTTTGCAGCAGAAAC
ACTGGGCAAGATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9524.80 Da
• Isoelectric Point: 4.2500

>AT179408 WP_162550505.1 NZ_CP063911:2872211-2872453 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDLAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT179408 NZ_CP084741:768484-768750 [Pectobacterium carotovorum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAATTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTATGACACACTTAATGACACTGATAAGCGCACGTTTGTACGCTTGTTACAAAGCGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGTGAACCGGAGGACGGAGAGCTGAAACGCATGATTTCCCTTATTCAGACGCGAAAT
AAAGATCGTGGCCCAGTGGCGATGTGA

Structures

Toxin

• AlphaFold DB: A0A1F2M041