Detailed information of TA system TA179395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 19789..21377
• Replicon: chromosome
• Accession: NZ_CP063911
• Organism: Klebsiella variicola strain M186-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0B7G636
• Locus tag: ISA80_RS00120
• Protein ID: WP_012968244.1
• Coordinates: 20055..21377 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MCN9
• Locus tag: ISA80_RS00115
• Protein ID: WP_008804920.1
• Coordinates: 19789..20055 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA80_RS00090	14940..15863	+	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-
ISA80_RS00095	15844..16998	-	1155	WP_008804924.1	FAD-dependent urate hydroxylase HpxO	-
ISA80_RS00100	17343..18722	+	1380	WP_012968246.1	purine permease	-
ISA80_RS00105	18700..19200	+	501	WP_023297604.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ISA80_RS00110	19197..19523	+	327	WP_008804921.1	hydroxyisourate hydrolase	-
ISA80_RS00115	19789..20055	+	267	WP_008804920.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ISA80_RS00120	20055..21377	+	1323	WP_012968244.1	type II toxin-antitoxin system HipA family toxin	Toxin
ISA80_RS00125	21394..22686	-	1293	WP_012968243.1	glycoside hydrolase family 10 protein	-
ISA80_RS00130	22819..23823	-	1005	WP_032730346.1	type 1 fimbrial protein	-
ISA80_RS00135	23811..26312	-	2502	WP_179153723.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48896.29 Da
• Isoelectric Point: 7.9109

>T179395 WP_012968244.1 NZ_CP063911:20055-21377 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGELMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T179395 NZ_CP084740:c482083-481826 [Dickeya zeae]
ATGCCTGAGATTGACAGACTGGCAGGGTTAATCTTCTATTTGTACTTTTACGATGATGAGCGGCACCACTTGCCGCATGT
GCATGTCTGCTACGGCAATCATGAACTTATCATCAGTATTCGTACCGGAGCCGAGATAGAAGGATATTTGCCAAATAACA
AACGTAAGCTGGCGATTGCACATATCATGACCCATCGTGAGCAACTGTTGAGTATGTGGGCACAGGCGGTCATCGGGAAG
AATCCGGGAAAATTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.30 Da
• Isoelectric Point: 8.5912

>AT179395 WP_008804920.1 NZ_CP063911:19789-20055 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT179395 NZ_CP084740:c481818-481333 [Dickeya zeae]
ATGCATAAGATTGTGGATGTTGACGTGATTGGCGATTTTTCGCTGCGCCTGACTTATGACGACGGCCTGATATGCGACGT
CAACCTGTATCACTATGCTTCACATCCTGTGTTTTCGAATGCCAGTCTGTTTGTCCGGTTCGGGTTATTACCTGATGGTT
CGTTGGAATGGCCGGATATCACGATTTCCTCGTCCGTATTACGGGAAAAGGGGGAAAATGTTGGCCATACGCTGGCGGTG
CGGGAGCGAAATTTTGCCGATATTATCTCCCGAGCCTTCTATGATGCCGTAATTGAGAACCGGCCAGAAATTCTGCAGGC
GGCATTGAAAATCGCAGTAGATAAACATGGCAACAGTACGGTGGCCAAAGAAGCGGGAGCCAAAAGTCGTACCAGCTTGT
ATAAGTCGCTGAATAAAAATACCAATGTCAGCCTGGCGACGGTGGTTAGCCTGGCCCACACGGTGTTGCAGTTGGAAGAG
AAATAG

Structures

Toxin

• AlphaFold DB: A0A0B7G636

Antitoxin

• AlphaFold DB: A0A1F2MCN9