Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4642951..4643570 Replicon chromosome
Accession NZ_CP063910
Organism Klebsiella variicola strain BS325-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA81_RS22465 Protein ID WP_002892050.1
Coordinates 4642951..4643169 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag ISA81_RS22470 Protein ID WP_008805436.1
Coordinates 4643196..4643570 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA81_RS22430 4638994..4639257 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
ISA81_RS22435 4639257..4639397 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA81_RS22440 4639394..4640092 - 699 WP_016160391.1 GNAT family N-acetyltransferase -
ISA81_RS22445 4640193..4641650 + 1458 WP_194387274.1 PLP-dependent aminotransferase family protein -
ISA81_RS22450 4641619..4642089 - 471 WP_008805434.1 YlaC family protein -
ISA81_RS22455 4642126..4642254 + 129 Protein_4385 hypothetical protein -
ISA81_RS22460 4642226..4642792 - 567 WP_008805435.1 maltose O-acetyltransferase -
ISA81_RS22465 4642951..4643169 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA81_RS22470 4643196..4643570 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
ISA81_RS22475 4644053..4647199 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
ISA81_RS22480 4647222..4648415 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179393 WP_002892050.1 NZ_CP063910:c4643169-4642951 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179393 NZ_CP084740:c335898-335584 [Dickeya zeae]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGGCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGCCGTCGACCGGATCGCCTTGTCC
CTGTCGTCAGGCTGACGGATGGCAAAGAATACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTAATCAGGTAAAGGCCGCAATAGATTTCCTCATTGACGGCATTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT179393 WP_008805436.1 NZ_CP063910:c4643570-4643196 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179393 NZ_CP084740:c336137-335901 [Dickeya zeae]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCACCCTGACAACCGCCCTGGATGCAGAACTTCGCCATCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAATCGTTTTCATGATGAACACGGCTGCTTCAGCGATGAATACCGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References