Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2546665..2547322 Replicon chromosome
Accession NZ_CP063910
Organism Klebsiella variicola strain BS325-2

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag ISA81_RS12425 Protein ID WP_002916310.1
Coordinates 2546665..2547075 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag ISA81_RS12430 Protein ID WP_002916312.1
Coordinates 2547056..2547322 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA81_RS12405 2542665..2544398 - 1734 WP_008806424.1 single-stranded-DNA-specific exonuclease RecJ -
ISA81_RS12410 2544404..2545117 - 714 WP_194386987.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ISA81_RS12415 2545140..2546036 - 897 WP_194386986.1 site-specific tyrosine recombinase XerD -
ISA81_RS12420 2546137..2546658 + 522 WP_008806427.1 flavodoxin FldB -
ISA81_RS12425 2546665..2547075 - 411 WP_002916310.1 protein YgfX Toxin
ISA81_RS12430 2547056..2547322 - 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
ISA81_RS12435 2547568..2548551 + 984 WP_043876073.1 tRNA-modifying protein YgfZ -
ISA81_RS12440 2548598..2549005 - 408 WP_131023732.1 hypothetical protein -
ISA81_RS12445 2549071..2549730 - 660 WP_043876072.1 hemolysin III family protein -
ISA81_RS12450 2549894..2550205 - 312 WP_012540591.1 N(4)-acetylcytidine aminohydrolase -
ISA81_RS12455 2550256..2550984 + 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T179388 WP_002916310.1 NZ_CP063910:c2547075-2546665 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T179388 NZ_CP084734:2762-3049 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT179388 WP_002916312.1 NZ_CP063910:c2547322-2547056 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT179388 NZ_CP084734:3036-3350 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References