Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1537193..1537783 Replicon chromosome
Accession NZ_CP063910
Organism Klebsiella variicola strain BS325-2

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag ISA81_RS07680 Protein ID WP_008804165.1
Coordinates 1537193..1537525 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag ISA81_RS07685 Protein ID WP_012541132.1
Coordinates 1537526..1537783 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA81_RS07655 1533231..1533476 + 246 WP_008804171.1 signal transduction protein PmrD -
ISA81_RS07660 1533607..1535061 + 1455 WP_008804170.1 AMP nucleosidase -
ISA81_RS07670 1535434..1536870 - 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
ISA81_RS07680 1537193..1537525 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISA81_RS07685 1537526..1537783 - 258 WP_012541132.1 antitoxin Antitoxin
ISA81_RS07690 1538312..1539412 + 1101 WP_110202033.1 phosphotransferase -
ISA81_RS07695 1539409..1540380 - 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
ISA81_RS07700 1540587..1541423 - 837 WP_008804155.1 alpha/beta hydrolase -
ISA81_RS07705 1541600..1542175 - 576 WP_043875014.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T179387 WP_008804165.1 NZ_CP063910:c1537525-1537193 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T179387 NZ_CP084733:448380-448769 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT179387 WP_012541132.1 NZ_CP063910:c1537783-1537526 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT179387 NZ_CP084733:448116-448373 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References