Detailed information of TA system TA179377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 126676..127346
• Replicon: plasmid pM212-2
• Accession: NZ_CP063909
• Organism: Klebsiella pneumoniae strain M212-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ISA82_RS26695
• Protein ID: WP_004213072.1
• Coordinates: 126676..127119 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ISA82_RS26700
• Protein ID: WP_004213073.1
• Coordinates: 127116..127346 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA82_RS26660	122085..122360	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
ISA82_RS26665	122423..122914	+	492	WP_011251327.1	DM13 domain-containing protein	-
ISA82_RS26670	122963..123883	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
ISA82_RS26675	123974..124380	+	407	Protein_135	GAF domain-containing protein	-
ISA82_RS26680	124895..125532	-	638	Protein_136	response regulator	-
ISA82_RS26685	125949..126242	+	294	Protein_137	transposase	-
ISA82_RS26690	126246..126662	+	417	Protein_138	restriction endonuclease	-
ISA82_RS26695	126676..127119	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA82_RS26700	127116..127346	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA82_RS26705	127954..129087	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ISA82_RS26710	129103..129396	+	294	WP_004213076.1	hypothetical protein	-
ISA82_RS26715	129386..129592	-	207	WP_004213077.1	hypothetical protein	-
ISA82_RS26720	129944..130234	+	291	WP_004213078.1	hypothetical protein	-
ISA82_RS26725	130224..131123	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..214844	214844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T179377 WP_004213072.1 NZ_CP063909:c127119-126676 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T179377 NZ_CP084723:c25350-24991 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGTCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTATGGAGATAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT179377 WP_004213073.1 NZ_CP063909:c127346-127116 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179377 NZ_CP084723:c24991-24692 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620