Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4230254..4230879 Replicon chromosome
Accession NZ_CP063908
Organism Klebsiella pneumoniae strain M212-2

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag ISA82_RS20395 Protein ID WP_012737091.1
Coordinates 4230496..4230879 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA82_RS20390 Protein ID WP_004150355.1
Coordinates 4230254..4230496 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA82_RS20365 4226245..4226844 + 600 WP_004151865.1 glucose-1-phosphatase -
ISA82_RS20370 4226838..4227698 + 861 WP_004146232.1 virulence factor BrkB family protein -
ISA82_RS20375 4227695..4228132 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA82_RS20380 4228177..4229118 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISA82_RS20385 4229132..4230049 - 918 WP_012737090.1 alpha/beta hydrolase -
ISA82_RS20390 4230254..4230496 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA82_RS20395 4230496..4230879 + 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA82_RS20400 4231053..4231982 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA82_RS20405 4231979..4232614 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA82_RS20410 4232611..4233513 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T179372 WP_012737091.1 NZ_CP063908:4230496-4230879 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179372 NZ_CP084714:c4759331-4758894 [Pseudomonas asiatica]
GTGATTCTGTGGCGTATCAGCGCCTATGCAGACTTGAGCGGAACGGGCGGCTTGCGTGTCAGCGGCCGCTGGCACCAGGC
GGGCCGGCCAGTGGTGTATGCCGCCACCAGCCCGGCCGGGGCGATGCTGGAGGTGCTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACCACCATGCGGCTGCTGCGTATCGAGTTGCCTGACACCGTTTCGCAAGCGCAGTTGCCCGCCTTGCAG
CCTGGCTGGTCGAGCCAGACCGAACTGACCCGGGGCCTGGGCAACCGCTTTCTGGATGAGTGTTCGGCGTTGCTGCTGCC
GGTGCCGAGCGCGATCATGCCCAGCACCACCAATTACCTTTTCAACCCGCGCCATCCGCAGGCAAACAGTGCCAGGCTCG
AGGTCGAGGACTTCACCCCGGACAGCCGGCTGTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179372 WP_004150355.1 NZ_CP063908:4230254-4230496 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179372 NZ_CP084714:c4759777-4759328 [Pseudomonas asiatica]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCATGCACTGCTGGAAATCCCCGAACT
CGCCAGTGACTTCGAAATCCACACCCGCATCACCCATGGCTTCGCTGCCACCTGGCTGGTGAAACTGACCGAACTCGGCG
TGTTCACCCCTGTGGAGCGTGACCAGATCATTCCCCTGCGCACGCTGAAAACCCGCATCGAACGTGACCAGCCGCTGACC
GTGGAAGAAAGCGACCGGCTGTTCCGTTCGGCCCATATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCAGGCAAGGC
AAAACGCTGGCTGTCCAAGCCCAAGGAGCGCTTCTCGGGGCTGACGCCGATGCAGATGCTAACCACCCAGCAGGGCACCA
CCCAGGTCGAAGAGATGTTGCTGCAGATCGCCGAGGGCTTCGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References