Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 37590..38209 Replicon chromosome
Accession NZ_CP063908
Organism Klebsiella pneumoniae strain M212-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag ISA82_RS00205 Protein ID WP_002892050.1
Coordinates 37590..37808 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag ISA82_RS00210 Protein ID WP_002892066.1
Coordinates 37835..38209 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA82_RS00170 33637..33900 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
ISA82_RS00175 33900..34040 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
ISA82_RS00180 34037..34735 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
ISA82_RS00185 34836..36287 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
ISA82_RS00190 36262..36732 - 471 WP_002892026.1 YlaC family protein -
ISA82_RS00195 36753..36893 + 141 WP_004147370.1 hypothetical protein -
ISA82_RS00200 36865..37431 - 567 WP_002892030.1 maltose O-acetyltransferase -
ISA82_RS00205 37590..37808 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
ISA82_RS00210 37835..38209 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
ISA82_RS00215 38695..41841 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
ISA82_RS00220 41864..43057 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T179359 WP_002892050.1 NZ_CP063908:c37808-37590 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T179359 NZ_CP084712:c2131406-2131038 [Sphingopyxis sp. USTB-05]
ATGATCGACGCGCAGTTCGATAGCGACATCCTGATCGACGCGCTGAACGGCATCGAAGCGGCGCGGGCCGAGATCCGCCG
CGCCGGGCGCAAGAGCGTGAGTCGTGTCAGCTGGACCGAGGTGATGTCAGCGGCCGACCCCACATCGGTGAAAGCGGTTG
AGGCCTTTCTCGGCTGCTTCCAGGTCGAGGAGATCGGGGATGCGGTCGCGCGTCGTGCCGCGGCGCTGCGCGCCGAGCGC
AAGGGCTTGACCTTGGCCGACGCTTTCGTACTGGCGACCGCACAGATCAGCGGGCGCATCCTGGTTACACGCAATATCAA
GGTGTTTCCGGCGTCGATGCCGGGTATTCGCGTTCCCTACACGCTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT179359 WP_002892066.1 NZ_CP063908:c38209-37835 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT179359 NZ_CP084712:c2131627-2131403 [Sphingopyxis sp. USTB-05]
ATGCGCTTTCTCGCGGACATCCCCGATGACGATATCCAGTGGCTCGACGCGCTTGCGGCCGAGCTAGGCGTGTCACGGGC
CGAGCTCGTTCGCCGTGCGGTAGCGGCCTATCGCGCGGACGTGTCAGGCGACGCGATCGATAACGCCTTCGGAATCTTTC
GCACCCGCGACGATATCGGCGACGGGCTGAAATATCAGCGGCGGCTTAGGGGCAAGCGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References