Detailed information of TA system TA179358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67027..67670
• Replicon: plasmid pBM327-1
• Accession: NZ_CP063907
• Organism: Klebsiella pneumoniae strain BM327-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: ISA83_RS26130
• Protein ID: WP_186474563.1
• Coordinates: 67254..67670 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: ISA83_RS26125
• Protein ID: WP_001261276.1
• Coordinates: 67027..67257 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA83_RS26085	62439..62552	+	114	WP_014343462.1	hypothetical protein	-
ISA83_RS26090	62681..62938	+	258	WP_194429505.1	hypothetical protein	-
ISA83_RS26095	62996..63775	-	780	WP_194429504.1	site-specific integrase	-
ISA83_RS26100	63973..64989	-	1017	WP_194429503.1	hypothetical protein	-
ISA83_RS26105	64986..65309	-	324	WP_004197641.1	hypothetical protein	-
ISA83_RS26110	65336..65737	-	402	WP_072199347.1	hypothetical protein	-
ISA83_RS26115	65905..66210	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	-
ISA83_RS26120	66212..66430	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	-
ISA83_RS26125	67027..67257	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA83_RS26130	67254..67670	+	417	WP_186474563.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA83_RS26135	67711..68589	-	879	WP_064182004.1	restriction endonuclease	-
ISA83_RS26140	69250..70527	+	1278	Protein_86	HlyD family secretion protein	-
ISA83_RS26145	70517..72625	+	2109	WP_114473038.1	peptidase domain-containing ABC transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	-	1..104836	104836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T179358 WP_186474563.1 NZ_CP063907:67254-67670 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T179358 NZ_CP084711:507789-508139 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATCAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT179358 WP_001261276.1 NZ_CP063907:67027-67257 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179358 NZ_CP084711:507503-507784 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023