Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65905..66430 Replicon plasmid pBM327-1
Accession NZ_CP063907
Organism Klebsiella pneumoniae strain BM327-1

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag ISA83_RS26115 Protein ID WP_004197633.1
Coordinates 65905..66210 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag ISA83_RS26120 Protein ID WP_006788213.1
Coordinates 66212..66430 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA83_RS26080 61037..61864 - 828 WP_194429506.1 replication initiation protein -
ISA83_RS26085 62439..62552 + 114 WP_014343462.1 hypothetical protein -
ISA83_RS26090 62681..62938 + 258 WP_194429505.1 hypothetical protein -
ISA83_RS26095 62996..63775 - 780 WP_194429504.1 site-specific integrase -
ISA83_RS26100 63973..64989 - 1017 WP_194429503.1 hypothetical protein -
ISA83_RS26105 64986..65309 - 324 WP_004197641.1 hypothetical protein -
ISA83_RS26110 65336..65737 - 402 WP_072199347.1 hypothetical protein -
ISA83_RS26115 65905..66210 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
ISA83_RS26120 66212..66430 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ISA83_RS26125 67027..67257 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ISA83_RS26130 67254..67670 + 417 WP_186474563.1 type II toxin-antitoxin system VapC family toxin -
ISA83_RS26135 67711..68589 - 879 WP_064182004.1 restriction endonuclease -
ISA83_RS26140 69250..70527 + 1278 Protein_86 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..104836 104836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T179357 WP_004197633.1 NZ_CP063907:c66210-65905 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T179357 NZ_CP084709:6990-7304 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT179357 WP_006788213.1 NZ_CP063907:c66430-66212 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT179357 NZ_CP084709:6724-7002 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References