Detailed information of TA system TA179354

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3389888..3390568
• Replicon: chromosome
• Accession: NZ_CP063906
• Organism: Klebsiella pneumoniae strain BM327-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ISA83_RS16495
• Protein ID: WP_174239183.1
• Coordinates: 3390254..3390568 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1X3RMB8
• Locus tag: ISA83_RS16490
• Protein ID: WP_064164243.1
• Coordinates: 3389888..3390211 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA83_RS16460	3385382..3386257	+	876	WP_032413278.1	GTPase family protein	-
ISA83_RS16465	3386470..3387171	+	702	WP_194429438.1	WYL domain-containing protein	-
ISA83_RS16470	3387177..3387824	+	648	WP_194429437.1	hypothetical protein	-
ISA83_RS16475	3387993..3388403	+	411	WP_102054331.1	hypothetical protein	-
ISA83_RS16480	3388513..3389334	+	822	WP_194429436.1	DUF945 domain-containing protein	-
ISA83_RS16485	3389405..3389875	+	471	WP_064164242.1	DNA repair protein RadC	-
ISA83_RS16490	3389888..3390211	+	324	WP_064164243.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ISA83_RS16495	3390254..3390568	+	315	WP_174239183.1	TA system toxin CbtA family protein	Toxin
ISA83_RS16500	3390682..3391515	+	834	WP_064164245.1	DUF4942 domain-containing protein	-
ISA83_RS16505	3391966..3392472	-	507	WP_019704403.1	YrhA family protein	-
ISA83_RS16510	3392483..3393616	-	1134	WP_032413284.1	HNH endonuclease	-
ISA83_RS16515	3393821..3394443	-	623	Protein_3212	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3372252..3398326	26074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-102)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11730.22 Da
• Isoelectric Point: 5.5157

>T179354 WP_174239183.1 NZ_CP063906:3390254-3390568 [Klebsiella pneumoniae]
ISTVPAKVPVSSRPSPVQVWQQLLTYLLDHHYGLTLNDTPFHDDGAIEEHIDAGITLADAVNFLVERYELVRIDRKGFSW
QEQTPFLTATDILRARRATGLTNT

Nucleotide

• Length: 315 bp

>T179354 NZ_CP084706:c50268-49798 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11968.77 Da
• Isoelectric Point: 5.5306

>AT179354 WP_064164243.1 NZ_CP063906:3389888-3390211 [Klebsiella pneumoniae]
MSITPPHEWGLQSDITPRFAARLVQEGNRLYYLADRAGLTGSFSPMHLQKLDLAFPLFVEQLEDMLCAGELNPHQQRQVK
IQLASLTCIADTLGSCGYVYISIYPTP

Nucleotide

• Length: 324 bp

>AT179354 NZ_CP084706:c50708-50265 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1X3RMB8