Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2837140..2837765 Replicon chromosome
Accession NZ_CP063906
Organism Klebsiella pneumoniae strain BM327-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ISA83_RS13890 Protein ID WP_002882817.1
Coordinates 2837382..2837765 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ISA83_RS13885 Protein ID WP_004150355.1
Coordinates 2837140..2837382 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA83_RS13860 2833130..2833729 + 600 WP_004151865.1 glucose-1-phosphatase -
ISA83_RS13865 2833723..2834583 + 861 WP_004146232.1 virulence factor BrkB family protein -
ISA83_RS13870 2834580..2835017 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
ISA83_RS13875 2835062..2836003 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ISA83_RS13880 2836018..2836935 - 918 WP_012737090.1 alpha/beta hydrolase -
ISA83_RS13885 2837140..2837382 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ISA83_RS13890 2837382..2837765 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ISA83_RS13895 2837939..2838868 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ISA83_RS13900 2838865..2839500 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ISA83_RS13905 2839497..2840399 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T179351 WP_002882817.1 NZ_CP063906:2837382-2837765 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T179351 NZ_CP084705:3162507-3162609 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT179351 WP_004150355.1 NZ_CP063906:2837140-2837382 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT179351 NZ_CP084705:c3162616-3162471 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References