Detailed information of TA system TA179346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 958560..959202
• Replicon: chromosome
• Accession: NZ_CP063906
• Organism: Klebsiella pneumoniae strain BM327-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ISA83_RS04870
• Protein ID: WP_075647414.1
• Coordinates: 959029..959202 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ISA83_RS04865
• Protein ID: WP_075647415.1
• Coordinates: 958560..958970 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA83_RS04825	953772..954206	-	435	WP_012542168.1	phage terminase small subunit P27 family	-
ISA83_RS04830	954456..954887	-	432	WP_081277328.1	hypothetical protein	-
ISA83_RS04835	954884..955207	-	324	WP_194429326.1	hypothetical protein	-
ISA83_RS04840	955159..955521	-	363	WP_017880264.1	HNH endonuclease	-
ISA83_RS04845	955505..955711	-	207	WP_023159883.1	hypothetical protein	-
ISA83_RS04850	956655..957005	-	351	WP_016160650.1	hypothetical protein	-
ISA83_RS04855	957002..957499	-	498	WP_075647416.1	lysozyme	-
ISA83_RS04860	957499..957714	-	216	WP_017880269.1	class II holin family protein	-
ISA83_RS04865	958560..958970	-	411	WP_075647415.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ISA83_RS04870	959029..959202	-	174	WP_075647414.1	type II toxin-antitoxin system HicA family toxin	Toxin
ISA83_RS04875	959468..960331	+	864	WP_075647413.1	hypothetical protein	-
ISA83_RS04880	960594..961010	+	417	WP_130943902.1	hypothetical protein	-
ISA83_RS04885	961026..961391	-	366	WP_075647411.1	DUF1133 family protein	-
ISA83_RS04890	961410..962390	-	981	WP_075647410.1	DUF968 domain-containing protein	-
ISA83_RS04895	962415..963224	-	810	WP_075647409.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
ISA83_RS04900	963221..964135	-	915	WP_040188691.1	conserved phage C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	925168..969201	44033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (86-128)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6603.75 Da
• Isoelectric Point: 11.3260

>T179346 WP_075647414.1 NZ_CP063906:c959202-959029 [Klebsiella pneumoniae]
VKQREFQRWLESQGVEITDGAKHLKLRLNGKRSVMPRHPGSEIVEQLRKAILKQLGL

Nucleotide

• Length: 174 bp

>T179346 NZ_CP084705:c1070305-1069922 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15269.40 Da
• Isoelectric Point: 4.4249

>AT179346 WP_075647415.1 NZ_CP063906:c958970-958560 [Klebsiella pneumoniae]
MRYPVNLSKAEEGGFFVEFPDIPEALTQGDTKEEALDNALDALVTAFEFYFEDGDRIPEPGVPTGDYVELPLSIEAKVLM
LNAFIESKLSQTELAAKMNVKKQEVTRLFDLKHSTKIDTIQKAVNSLGHRMELKYA

Nucleotide

• Length: 411 bp

>AT179346 NZ_CP084705:c1070547-1070305 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA