Detailed information of TA system TA179336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 210060..210730
• Replicon: plasmid pBM334-2
• Accession: NZ_CP063905
• Organism: Klebsiella pneumoniae strain BM334-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: ISA84_RS27455
• Protein ID: WP_004213072.1
• Coordinates: 210287..210730 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: ISA84_RS27450
• Protein ID: WP_004213073.1
• Coordinates: 210060..210290 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA84_RS27425	206283..207182	-	900	WP_004225022.1	nucleotide-binding protein	-
ISA84_RS27430	207172..207462	-	291	WP_004213078.1	hypothetical protein	-
ISA84_RS27435	207814..208020	+	207	WP_004213077.1	hypothetical protein	-
ISA84_RS27440	208010..208303	-	294	WP_004213076.1	hypothetical protein	-
ISA84_RS27445	208319..209452	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
ISA84_RS27450	210060..210290	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ISA84_RS27455	210287..210730	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
ISA84_RS27460	210744..211160	-	417	Protein_228	restriction endonuclease	-
ISA84_RS27465	211164..211457	-	294	Protein_229	transposase	-
ISA84_RS27470	211874..212512	+	639	Protein_230	mucoid phenotype A regulator RmpA	-
ISA84_RS27475	213027..213433	-	407	Protein_231	GAF domain-containing protein	-
ISA84_RS27480	213524..214444	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
ISA84_RS27485	214493..214984	-	492	WP_011251327.1	DM13 domain-containing protein	-
ISA84_RS27490	215047..215322	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA	1..226993	226993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T179336 WP_004213072.1 NZ_CP063905:210287-210730 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T179336 NZ_CP084704:c2418313-2417906 [Desulfurispirillum indicum]
ATGCTTCGCTACCTGTTAGACACCAGCATCGTTATCTATGTCATCAAGCGTCGGCCCATTGAATTGCTGGGGAAATTCAA
CGAAGAAACTGGCCGGATGGCTATCAGCAGCATCACCCTGGCCGAGTTGATGCACGGCGTCGAAAAAAGCGCCCAGCCCA
CAAAAAACCTCCAGCATGTGGAAGACTTCATTTCCCGCTTGGAAGTGCTGCCATACGCAGCTGCGGCAGCGGTGCATTAC
GGCCATATTCGGGCCACCCTGGAGAGACAGGGAATTCCCATAGGAAGTAACGACCTCCATATCGCTGCCCACAGTCGCAG
CGCGGGACTCATCCTGGTGACAAACAACGTTAGGGAGTTTGAGCGAGTGCCCGGCCTGCTCTATGAAAACTGGCTTACAG
GATTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT179336 WP_004213073.1 NZ_CP063905:210060-210290 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT179336 NZ_CP084704:c2418546-2418313 [Desulfurispirillum indicum]
ATGGCTACTGGAACCGTATTTGAAAACAACCGAACTCAGGCTGTCAGGCTCCCAGCGGAAACCAGGTTCCCAGAGGGCGT
GAAGAAAGTGCACATCCGCGTACATGGCAACGAGCGTATTCTCAGCCCCCAGAATGCTGCCTGGGACTCTTTTTTCAATG
CCTCGGAAAATGTCAGCGATGACTTCATGGAAGAGCGTGCCAGCCAATCCCCACAGGAACGTGAGGAGCTGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620