Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 48477..49213 Replicon plasmid pBM334-2
Accession NZ_CP063905
Organism Klebsiella pneumoniae strain BM334-2

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag ISA84_RS26570 Protein ID WP_004098919.1
Coordinates 48731..49213 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag ISA84_RS26565 Protein ID WP_004213599.1
Coordinates 48477..48743 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA84_RS26540 43709..44266 + 558 WP_004213590.1 recombinase family protein -
ISA84_RS26545 44269..47238 + 2970 WP_004213592.1 Tn3 family transposase -
ISA84_RS26550 47280..47774 + 495 WP_004213594.1 hypothetical protein -
ISA84_RS26555 47835..48038 + 204 WP_004213596.1 hemolysin expression modulator Hha -
ISA84_RS26560 48052..48282 + 231 WP_004213598.1 hypothetical protein -
ISA84_RS26565 48477..48743 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
ISA84_RS26570 48731..49213 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
ISA84_RS26575 49634..50861 + 1228 Protein_51 IS3 family transposase -
ISA84_RS26580 50857..51252 - 396 Protein_52 DDE-type integrase/transposase/recombinase -
ISA84_RS26585 51427..52449 - 1023 WP_004214536.1 porphobilinogen synthase -
ISA84_RS26590 52513..53859 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA 1..226993 226993
- inside IScluster/Tn - - 43709..50861 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T179335 WP_004098919.1 NZ_CP063905:48731-49213 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T179335 NZ_CP084704:c1071313-1071014 [Desulfurispirillum indicum]
ATGACCTATAAGCTGCGCTTTCACGAAAAAGCCCTTGCCGAATGGCGCGCACTGGACAACAGTATTCGCGAACCCCTCAA
AAGAAAACTTGCCAGCCGACTGCAACAGCCCCATGTTCCTGCCGATGCCCTCAGCGGCATGCCCAACTGTTATAAAATCA
AACTCAAACGCATTGGATATCGCCTGGTATATCAGGTCTGCAACGACACCGTAGTGGTATCTGTGATCGCGGTAGGCAAG
CGCGACAAAAACCATGTTTATGGTAGCACCAATACTCGGCTGGAAACGGACAAGCCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT179335 WP_004213599.1 NZ_CP063905:48477-48743 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT179335 NZ_CP084704:c1071548-1071303 [Desulfurispirillum indicum]
ATGGAATCCATCCTGGCAGATGCAACGGTGAGCGTAACGGAACTCAAGCGCAGCTATGCTGAAATCCTGCGAAAGGCTGA
GGACTTCCCCGTAGCCATCCTGAATCATAATCGCCCGGAAGCCTACCTGCTGCCTGCGGCCTATTATGAACGGCTTTTGG
AGCGTCTGGAAGATATCGAAGATGCTTTACTGATACGAGAGCGACAAGAGGGGCCGTTTATTGAGGTAAGCCTCGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References