Detailed information of TA system TA179329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 3437398..3438735
• Replicon: chromosome
• Accession: NZ_CP063904
• Organism: Klebsiella pneumoniae strain BM334-2

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: ISA84_RS16780
• Protein ID: WP_032424556.1
• Coordinates: 3437800..3438735 (+)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: ISA84_RS16775
• Protein ID: WP_023302769.1
• Coordinates: 3437398..3437793 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISA84_RS16745	3432642..3432932	+	291	WP_022615704.1	hypothetical protein	-
ISA84_RS16750	3432997..3433902	+	906	WP_022615703.1	DUF1738 domain-containing protein	-
ISA84_RS16755	3434095..3434466	+	372	WP_022615702.1	hypothetical protein	-
ISA84_RS16760	3434640..3436235	+	1596	WP_023302771.1	TraI domain-containing protein	-
ISA84_RS16765	3436407..3436871	+	465	WP_022615700.1	GNAT family N-acetyltransferase	-
ISA84_RS16770	3436877..3437143	-	267	WP_023302770.1	hypothetical protein	-
ISA84_RS16775	3437398..3437793	+	396	WP_023302769.1	hypothetical protein	Antitoxin
ISA84_RS16780	3437800..3438735	+	936	WP_032424556.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
ISA84_RS16785	3438743..3439432	-	690	WP_023302767.1	hypothetical protein	-
ISA84_RS16790	3439632..3440426	-	795	WP_023302766.1	RES domain-containing protein	-
ISA84_RS16795	3440845..3442524	-	1680	WP_004212561.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-298)

Antitoxin

• Protein of unknown function DUF6088 (7-109)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 35389.66 Da
• Isoelectric Point: 5.6165

>T179329 WP_032424556.1 NZ_CP063904:3437800-3438735 [Klebsiella pneumoniae]
MLFSDVTDALGIESGAIVEKDHYIVELLHLLKPLTFDTHQLLFAGGTALAKSGIVLNRMSEDVDIKLVPTDTFLSLSRTQ
RKGIRKEIVQTISHAITATDMFSFDDAHPKVTRDEYRYNDIPVRYPQRVAQAPCLRPFIQLELMEANLLEAPEFRDIHTL
VTTLTGKAPVISAFPCATIISTQAEKLVAMLRRTAAVARNIERQDDASLVRHIYDTYCIVQAGNVDMCLLTDFFKQTIEQ
DIQRYGNQYPQFCVSPNDELKMGLDELEHNPLYKTRYQQFVAPMVYGARKVSWDEAYASFRQMALIILDTY

Nucleotide

• Length: 936 bp

>T179329 NZ_CP084699:c61648-61220 [Rhizobium ruizarguesonis]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACATGTCCTGCAATGGCTGGA
CGATCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GTCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAACGCTTCGAGCAGCGTGTCATCCCTGTAGAC
CAGGCGGTTGCGATAACCTGGGGAGACTTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGGTGGCTT
GATTGCCGCCACCGCGATTGCCCACGATCTCACCCTCGCCACCCGTAATATAAAGGATTTCGAGAGCCTTGGGATCGAGC
TTGTCGATCCTTGGATCGAGCGCCCGTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14800.08 Da
• Isoelectric Point: 11.1078

>AT179329 WP_023302769.1 NZ_CP063904:3437398-3437793 [Klebsiella pneumoniae]
MTIKTRIQSRLKRSKRYVFTRDDFKDIAGYDQIGRVLRDLLKEGQLLKVGYGVYTKARRNSITGKVMPAAPRGSSAVIVE
TLERLKVRYRYEGATQTYNSGESTQIPVSPEIKTSPRFKRALSVGNSKLNG

Nucleotide

• Length: 396 bp

>AT179329 NZ_CP084699:c61911-61645 [Rhizobium ruizarguesonis]
ATGCAGCCATCCCGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACACCGCAAACGATCATGCGCAATGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCCGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTGATCTCCAGCTGTGA