179328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3383328..3384138 Replicon chromosome
Accession NZ_CP063904
Organism Klebsiella pneumoniae strain BM334-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag ISA84_RS16535 Protein ID WP_004178461.1
Coordinates 3383328..3383861 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag ISA84_RS16540 Protein ID WP_002887278.1
Coordinates 3383872..3384138 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISA84_RS16530 3382159..3383280 + 1122 WP_004214138.1 cupin domain-containing protein -
ISA84_RS16535 3383328..3383861 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
ISA84_RS16540 3383872..3384138 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
ISA84_RS16545 3384241..3385674 - 1434 WP_004214141.1 Cu(+)/Ag(+) sensor histidine kinase -
ISA84_RS16550 3385664..3386347 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
ISA84_RS16555 3386519..3387904 + 1386 WP_004214145.1 efflux transporter outer membrane subunit -
ISA84_RS16560 3387922..3388266 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T179328 WP_004178461.1 NZ_CP063904:c3383861-3383328 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T179328 NZ_CP084697:c454700-454407 [Rhizobium ruizarguesonis]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGAAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCGGG
CCAGGCAAAGCGATACCACGATGAACTTTTCGCATTATTTGATCTGATAGCTGTCAATCCACGCTTCGCGCGCGAACGGG
ATGAGATTGATCCGCCCGTCAGAATTCATCCCTTCAAAGCGCATCTAATCGTTTATCGGATCGAAGATGATGAGACAATT
TTTGTAGTACGAATTCGCCACGCTCATGAAGATTGGGCAAGTGACTCAATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT179328 WP_002887278.1 NZ_CP063904:c3384138-3383872 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT179328 NZ_CP084697:c454939-454697 [Rhizobium ruizarguesonis]
ATGGCCACGATGAACGTATCTCTTCCAGGCCCGATGAAGGATTGGGTGGAGGACCAGGCCAGGACCGGGCGATATTCGAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTGTCGGCATCCGATCAAAGGATGAACTCTTTGCTGAGGCAATGGCTCGTGCCAACAAGTCA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References